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dc.contributor.authorThunemann, Martinen_US
dc.contributor.authorLu, Y.en_US
dc.contributor.authorLiu, X.en_US
dc.contributor.authorKilic, K.en_US
dc.contributor.authorDesjardins, M.en_US
dc.contributor.authorVandenberghe, M.en_US
dc.contributor.authorSadegh, S.en_US
dc.contributor.authorSaisan, P. A.en_US
dc.contributor.authorCheng, Q.en_US
dc.contributor.authorWeldy, K. L.en_US
dc.contributor.authorLyu, H.en_US
dc.contributor.authorDjurovic, S.en_US
dc.contributor.authorAndreassen, O. A.en_US
dc.contributor.authorDale, A. M.en_US
dc.contributor.authorDevor, Annaen_US
dc.contributor.authorKuzum, D.en_US
dc.date.accessioned2020-12-17T18:50:29Z
dc.date.available2020-12-17T18:50:29Z
dc.date.issued2018
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/29789548
dc.identifier.citationM. Thunemann, Y. Lu, X. Liu, K. Kilic, M. Desjardins, M. Vandenberghe, S. Sadegh, P.A. Saisan, Q. Cheng, K.L. Weldy, H. Lyu, S. Djurovic, O.A. Andreassen, A.M. Dale, A. Devor, D. Kuzum. 2018. "Deep 2-photon imaging and artifact-free optogenetics through transparent graphene microelectrode arrays." Nat Commun, Volume 9, Issue 1, pp. 2035 - ?. https://doi.org/10.1038/s41467-018-04457-5
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/2144/41820
dc.description.abstractRecent advances in optical technologies such as multi-photon microscopy and optogenetics have revolutionized our ability to record and manipulate neuronal activity. Combining optical techniques with electrical recordings is of critical importance to connect the large body of neuroscience knowledge obtained from animal models to human studies mainly relying on electrophysiological recordings of brain-scale activity. However, integration of optical modalities with electrical recordings is challenging due to generation of light-induced artifacts. Here we report a transparent graphene microelectrode technology that eliminates light-induced artifacts to enable crosstalk-free integration of 2-photon microscopy, optogenetic stimulation, and cortical recordings in the same in vivo experiment. We achieve fabrication of crack- and residue-free graphene electrode surfaces yielding high optical transmittance for 2-photon imaging down to ~ 1 mm below the cortical surface. Transparent graphene microelectrode technology offers a practical pathway to investigate neuronal activity over multiple spatial scales extending from single neurons to large neuronal populations.en_US
dc.format.extentp. 2035en_US
dc.language.isoen_US
dc.relation.ispartofNat Commun
dc.rights© The Author(s) 2018. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleDeep 2-photon imaging and artifact-free optogenetics through transparent graphene microelectrode arraysen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1038/s41467-018-04457-5
pubs.elements-sourcemanual-entryen_US
pubs.notesThunemann, Martin Lu, Yichen Liu, Xin Kilic, Kivilcim Desjardins, Michele Vandenberghe, Matthieu Sadegh, Sanaz Saisan, Payam A Cheng, Qun Weldy, Kimberly L Lyu, Hongming Djurovic, Srdjan Andreassen, Ole A Dale, Anders M Devor, Anna Kuzum, Duygu eng R01 MH111359/MH/NIMH NIH HHS/ R01 NS057198/NS/NINDS NIH HHS/ S10 RR029050/RR/NCRR NIH HHS/ U01 NS094232/NS/NINDS NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't England Nat Commun. 2018 May 23;9(1):2035. doi: 10.1038/s41467-018-04457-5. Recent advances in optical technologies such as multi-photon microscopy and optogenetics have revolutionized our ability to record and manipulate neuronal activity. Combining optical techniques with electrical recordings is of critical importance to connect the large body of neuroscience knowledge obtained from animal models to human studies mainly relying on electrophysiological recordings of brain-scale activity. However, integration of optical modalities with electrical recordings is challenging due to generation of light-induced artifacts. Here we report a transparent graphene microelectrode technology that eliminates light-induced artifacts to enable crosstalk-free integration of 2-photon microscopy, optogenetic stimulation, and cortical recordings in the same in vivo experiment. We achieve fabrication of crack- and residue-free graphene electrode surfaces yielding high optical transmittance for 2-photon imaging down to ~ 1 mm below the cortical surface. Transparent graphene microelectrode technology offers a practical pathway to investigate neuronal activity over multiple spatial scales extending from single neurons to large neuronal populations.en_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
dc.identifier.orcid0000-0003-4139-079X (Thunemann, M)
dc.identifier.orcid0000-0002-5143-3960 (Devor, A)
dc.identifier.mycv530080


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© The Author(s) 2018. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Except where otherwise noted, this item's license is described as © The Author(s) 2018. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/