Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope
Vickers, Nicholas A.
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Citation (published version)Peng Lin, Hongli Ni, Huate Li, Nicholas A Vickers, Yuying Tan, Ruyi Gong, Thomas Bifano, Ji-Xin Cheng. 2020. "Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope." Optics Express, Volume 28, Issue 20, pp. 30210 - 30210. https://doi.org/10.1364/oe.404869
Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.
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