A comparison of the sealing ability and biocompatibility of retrograde filling materials
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The purpose of this study was to compare the effectiveness of the apical seal obtained by retrograde obturation of the rootcanal system with zinc amalgam, zinc-free amalgam, Cavitand Stailine Super EBA Cement. The present investigation also examined the inflammatory response of rat dorsal subcutaneous connective tissue to the mentioned materials. The first objective was evaluated in vitro by means of a dye penetration study. Retrograde filling of the root canal system of extracted non-carious single rooted human teeth with the test materials was performed. After 27 days, the teeth were submerged into dye solution for 72 hours and each specimen, beginning at the apical portion, was sequentially sectioned. The extent of dye penetration was assessed and the results were statistically compared using a simple analysis of variance. The second objective was evaluated in vivo by means of a screening secondary test. Teflon tubes containing the test materials were implanted into the subcutaneous connective tissue of four Sprague-Dawley rats. The interface between the test materials and the connective tissue was examined after 2, 15, 30 and 90 days and the tissue reactions were classified as mild, moderate and severe. The following conclusions were drawn: 1) Zinc amalgam and zinc-free amalgam afforded a significantly better seal than Cavitor Stailine. 2) No significant difference was found between the sealing ability of zinc amalgam and zinc-free amalgam, nor between Cavit and Stailine. 3) Unfilled specimens exhibited complete dye penetration. 4) Debridement and sanitization, and tridimensional obturation of the root canal system should be attempted prior to placement of a retrograde filling. 5) Of the examined test materials, zinc-free and Cavit exhibited the mildest inflammatory response after 2, 15, and 30 day time-periods. 6) After 90 days of implantation, mild inflammatory tissue reactions were observed with all test materials. 7) Placement of test materials in Teflon tubes during implantation tests controls the quantity and form, and prevents the materials from disintegration. 8) A sterile, inert hollow tube does not cause inflammation to the surrounding tissues.
Colored photographs included.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1983 (Endodontics)Bibliography: leaves 206-224.
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