Quantification and characterization of collagen at various ages from rabbit and human dental pulp tissue
Pranikoff, Howard Leo
MetadataShow full item record
Collagen from the human and rabbit dental pulp tissue was studied as a function of age. The two parameters looked at were solubility and the amount of collagen present as compared to total protein. Negligible amounts of collagen were soluble in 1 M NaCl or O.5 N acetic acid. Pepsin solubilized the greatest amount of the soluble collagen. The solubility decreased with age in the human samples, but there was a a slight increase in solubility in the older rabbit group. In both human and rabbit samples, the insoluble residue was typical of relatively pure collagen and the data suggests, though not conclusively, that this fraction is predominantly Type I collagen. There were, however, slight differences in the amino acid ana1yses between the human and rabbit samples. The human pulp tissue was composed of three to four times as much collagen as was the rabbit tissue. The percentage of collagen in the human pulp increased with age, whereas, the rabbit sample did not demonstrate any significant change. Macroscopically, the human pulps appeared more fibrous than the rabbit tissue, and this was more pronounced with age. Also, the older human pulps exhibited calcified deposits and none were seen in the rabbit pulps.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1979 (Endodontics)Bibliography : p. 107-117.
RightsThis work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.