The effect of osteoblast addition to critical defect and the effect of D4 and D5 resolvins to calvaria
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OBJECTIVE: A calvarial defect model was used to study the effect of additions of osteoblasts in both a grid culture and implantation onto chick embryo membrane. The secondary aim was to study the effects of resolvins D4 and D5, parathyroid hormone and ascorbic acid on collagen synthesis and cell differentiation. METHODS: A mouse calvaria critical defect model was used in both an in-vitro and ex-vivo cultures. Calcium uptake was measured by an Arsenazo III microplate assay. Neutral Red and H&E staining were used for histological analysis. RESULTS: There was no significant difference is observed between groups supplemented with osteoblasts and those without. Addition of D4 and D5 decreased macrophage numbers in NR-stained calvaria. DISCUSSION: Osteoblasts require a specific environment and nutrition in order to survive and synthesize bone matrix in a defect or injured area. D4 and D5 resolvins may be able to play a role clinically in pro-resolution of inflammation post-surgically.