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dc.contributor.authorGrazon, C.en_US
dc.contributor.authorBaer, R.C.en_US
dc.contributor.authorKuzmanović, U.en_US
dc.contributor.authorNguyen, T.en_US
dc.contributor.authorChen, M.en_US
dc.contributor.authorZamani, M.en_US
dc.contributor.authorChern, M.en_US
dc.contributor.authorAquino, P.en_US
dc.contributor.authorZhang, X.en_US
dc.contributor.authorLecommandoux, S.en_US
dc.contributor.authorFan, Andyen_US
dc.contributor.authorCabodi, Marioen_US
dc.contributor.authorKlapperich, Catherineen_US
dc.contributor.authorGrinstaff, Mark W.en_US
dc.contributor.authorDennis, Allison M.en_US
dc.contributor.authorGalagan, James E.en_US
dc.coverage.spatialEnglanden_US
dc.date2020-02-03
dc.date.accessioned2021-11-03T15:35:12Z
dc.date.available2021-11-03T15:35:12Z
dc.date.issued2020-03-09
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/32152281
dc.identifier.citationC. Grazon, R.C. Baer, U. Kuzmanović, T. Nguyen, M. Chen, M. Zamani, M. Chern, P. Aquino, X. Zhang, S. Lecommandoux, A. Fan, M. Cabodi, C. Klapperich, M.W. Grinstaff, A.M. Dennis, J.E. Galagan. 2020. "A progesterone biosensor derived from microbial screening.." Nat Commun, Volume 11, Issue 1, pp. 1276 - ?. https://doi.org/10.1038/s41467-020-14942-5
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/2144/43262
dc.description.abstractBacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe an approach to identify and isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte and to develop these TFs into biosensor devices. Our approach utilizes a combination of genomic screens and functional assays to identify and isolate biosensing TFs, and a quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing analyte recognition into real-time quantitative measurements. We use this approach to identify a progesterone-sensing bacterial aTF and to develop this TF into an optical sensor for progesterone. The sensor detects progesterone in artificial urine with sufficient sensitivity and specificity for clinical use, while being compatible with an inexpensive and portable electronic reader for point-of-care applications. Our results provide proof-of-concept for a paradigm of microbially-derived biosensors adaptable to inexpensive, real-time sensor devices.en_US
dc.description.sponsorshipU54 EB015403 - NIBIB NIH HHS; KL2 TR001411 - NCATS NIH HHS; S10 OD016326 - NIH HHSen_US
dc.format.extentp. 1276en_US
dc.languageeng
dc.language.isoen_US
dc.relation.ispartofNat Commun
dc.rights© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleA progesterone biosensor derived from microbial screeningen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1038/s41467-020-14942-5
pubs.elements-sourcepubmeden_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Chemistryen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.publication-statusPublished onlineen_US
dc.identifier.mycv552174


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© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.