Evaluation of gastrointestinal oxidation status as a predictor for pediatric inflammatory bowel disease activity
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INTRODUCTION: Inflammatory bowel disease (IBD) is a growing public health concern with a pressing need for new diagnostic and disease activity biomarkers. Recently, studies have linked IBD disease factors to an imbalance in the gut’s reductive-oxidative (redox) defensive mechanisms and the resulting oxidative stress due to reactive oxygen species. With this new paradigm, direct measurement of redox status in the body could be utilized as a novel biomarker of disease activity for patients with IBD. OBJECTIVES: The goal of this study is to determine how gastrointestinal oxidative state relates to IBD disease activity. Additionally, this study aims to establish a reproducible and accurate measurement protocol for measuring redox status in the human body. METHODS: Patients with and without IBD admitted to Boston Children’s Hospital (Boston, MA) were enrolled in the study from October 2020 to February 2021. Stool and urine samples were collected from these patients and the oxidative status in these biosamples was measured via three redox measuring systems. RESULTS: Data suggests that relative stool oxidative state is more positive in patients with active disease states compared to controls. Also, a finalized protocol for the measurement of relative redox status in stool and urine was established in this study. CONCLUSION: With a reliable and accurate method of measurement established, the potential for relative redox status in the human body to serve as a predictive biomarker for IBD state is promising. Moving forward this study will focus on expanding the study’s size and types of samples to make more significant conclusions in the future. The usefulness of oxidative state in the body as a disease biomarker is just beginning to be realized.