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dc.contributor.authorFan, Xuepingen_US
dc.contributor.authorLi, Qinggangen_US
dc.contributor.authorPisarek-Horowitz, Annaen_US
dc.contributor.authorRasouly, Hila Miloen_US
dc.contributor.authorWang, Xianglingen_US
dc.contributor.authorBonegio, Ramon G.en_US
dc.contributor.authorWang, Hangen_US
dc.contributor.authorMcLaughlin, Margareten_US
dc.contributor.authorMangos, Steveen_US
dc.contributor.authorKalluri, Raghuen_US
dc.contributor.authorHolzman, Lawrence B.en_US
dc.contributor.authorDrummond, Iain A.en_US
dc.contributor.authorBrown, Dennisen_US
dc.contributor.authorSalant, David J.en_US
dc.contributor.authorLu, Weiningen_US
dc.date.accessioned2012-11-07T20:30:22Z
dc.date.available2012-11-07T20:30:22Z
dc.date.issued2012-07-26
dc.identifier.citationFan, Xueping, et al. Inhibitory Effects of Robo2 on Nephrin: A Crosstalk between Positive and Negative Signals Regulating Podocyte Structure. Reports 2, 52–61, July 26, 2012. http://dx.doi.org/10.1016/j.celrep.2012.06.002
dc.identifier.otherdoi:10.1016/j.celrep.2012.06.002
dc.identifier.urihttp://dx.doi.org/10.1016/j.celrep.2012.06.002
dc.identifier.urihttps://hdl.handle.net/2144/4364
dc.description.abstractRobo2 is the cell surface receptor for the repulsive guidance cue Slit and is involved in axon guidance and neuronal migration. Nephrin is a podocyte slit- diaphragm protein that functions in the kidney glomerular filtration barrier. Here, we report that Robo2 is expressed at the basal surface of mouse podocytes and colocalizes with nephrin. Biochemical studies indicate that Robo2 forms a complex with nephrin in the kidney through adaptor protein Nck. In contrast to the role of nephrin that promotes actin polymerization, Slit2-Robo2 signaling inhibits nephrin-induced actin polymerization. In addition, the amount of F-actin associated with nephrin is increased in Robo2 knockout mice that develop an altered podocyte foot process structure. Genetic interaction study further reveals that loss of Robo2 alleviates the abnormal podocyte structural pheno- type in nephrin null mice. These results suggest that Robo2 signaling acts as a negative regulator on neph- rin to influence podocyte foot process architecture.en_US
dc.description.sponsorshipThis work is supported by NIH grants R01DK078226 (to W.L.) and R01DK30932 (to D.J.S.), and is also supported in part by Research Grant #6-FY08-304 from the March of Dimes Foundation (to W.L.).en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.rightsAttribution 3.0 Unporteden_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.titleInhibitory Effects of Robo2 on Nephrin: A Crosstalk between Positive and Negative Signals Regulating Podocyte Structureen_US
dc.typeArticleen_US


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