Drug induced gingival hyperplasia
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Drug induced gingival hyperplasia is a side effect of certain drugs taken systemically which have an effect intraorally and can affect function and esthetics. In this study we tried to find an explanation at the molecular level. We know from previous experiments that lysyl oxidase is responsible for the cross-linking of collagen. Basic fibroblast growth factor down regulates mRNA levels of lysyl oxidase. The goal of this study was to determine whether drugs (cyclosporin A, dilantin, and nifedipine) block this down regulation allowing increased deposition of ground substance in the extracellular matrix leading to fibrosis. Gingival fibroblasts were treated with nifedipine plus or minus basic fibroblast growth factor. RNA was isolated and changes in lysyl oxidase, collagen, and elastin mRNA levels were measured. The lysyl oxidase enzyme activity was also measured to see if it was concordant with the mRNA levels. Down regulation by basic fibroblast growth factor was reproducible at high concentrations of basic fibroblast growth factor only (1 nM). At 0.1 nM this down regulation was not consistant anymore. The blocking effect produced by the drugs was not reproducible at high or low concentrations of basic growth factor. This may be due to the stage at which the cells were treated, the kind of cells, or the time of treatment.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1998 (Periodontology and Oral Biology).Includes bibliographical references: (leaves 31-35).
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