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dc.contributor.authorBlanke, Nathanen_US
dc.contributor.authorGo, Veronicaen_US
dc.contributor.authorRosene, Douglas L.L.en_US
dc.contributor.authorBigio, Irving J.en_US
dc.date.accessioned2022-05-12T18:54:20Z
dc.date.available2022-05-12T18:54:20Z
dc.date.issued2021-03-22
dc.identifier.citationNathan Blanke, Veronica Go, Douglas L. L. Rosene, and Irving J. Bigio "Quantitative birefringence microscopy for imaging the structural integrity of CNS myelin following circumscribed cortical injury in the rhesus monkey," Neurophotonics 8(1), 015010 (22 March 2021). https://doi.org/10.1117/1.NPh.8.1.015010
dc.identifier.issn2329-423X
dc.identifier.urihttps://hdl.handle.net/2144/44402
dc.description.abstractSignificance: Myelin breakdown is likely a key factor in the loss of cognitive and motor function associated with many neurodegenerative diseases. Aim: New methods for imaging myelin structure are needed to characterize and quantify the degradation of myelin in standard whole-brain sections of nonhuman primates and in human brain. Approach: Quantitative birefringence microscopy (qBRM) is a label-free technique for rapid histopathological assessment of myelin structural breakdown following cortical injury in rhesus monkeys. Results: We validate birefringence microscopy for structural imaging of myelin in rhesus monkey brain sections, and we demonstrate the power of qBRM by characterizing the breakdown of myelin following cortical injury, as a model of stroke, in the motor cortex. Conclusions: Birefringence microscopy is a valuable tool for histopathology of myelin and for quantitative assessment of myelin structure. Compared to conventional methods, this label-free technique is sensitive to subtle changes in myelin structure, is fast, and enables more quantitative assessment, without the variability inherent in labeling procedures such as immunohistochemistry.en_US
dc.language.isoen_US
dc.publisherSociety of Photo-optical Instrumentation Engineersen_US
dc.relation.ispartofNeurophotonics
dc.rights© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleQuantitative birefringence microscopy for imaging the structural integrity of CNS myelin following circumscribed cortical injury in the rhesus monkeyen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1117/1.NPh.8.1.015010
pubs.elements-sourcemanual-entryen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.organisational-groupBoston University, School of Medicineen_US
pubs.publication-statusPublisheden_US
dc.identifier.mycv618123


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© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Except where otherwise noted, this item's license is described as © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.