The in vitro metabolism of 17-hydroxy-11-desoxycorticoserone (Reichstein's compound S).
Forchielli, Enrico H.
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Although it has been known that ∆4-pregnene-17a, 21-diol- 3, 20-dione (Reichstein's compoundS) is an intermediate in the biosynthesis of hydrocortisone (13), little was known of other aspects of its metabolism. The in vitro technique for study was chosen in the hope that the isolation of metabolites would permit an interpretation of the mechanism involved in the metabolism of compoundS. Since it has been demonstrated that liver contains a relatively high concentration of the necessary enzyme systems for the transformation of steroids, preparations of this tissue were selected for this in vitro metabolism study. Compound S was incubated with several types of enzyme preparations of rat liver, and products containing reduced structural configurations involving positions 3 and 5 were isolated. The utilization of silica gel column and paper chromatographic resolution has permitted the purification and resolution of two steroid metabolites in crystalline form. [TRUNCATED]
Thesis (M.A.)--Boston University