Deep 2-photon imaging and artifact-free optogenetics through transparent graphene microelectrode arrays
Date
2018
Authors
Thunemann, Martin
Lu, Y.
Liu, X.
Kilic, K.
Desjardins, Michèle
Vandenberghe, M.
Sadegh, S.
Saisan, P. A.
Cheng, Qun
Weldy, K. L.
Version
Published version
OA Version
Citation
M. Thunemann, Y. Lu, X. Liu, K. Kilic, M. Desjardins, M. Vandenberghe, S. Sadegh, P.A. Saisan, Q. Cheng, K.L. Weldy, H. Lyu, S. Djurovic, O.A. Andreassen, A.M. Dale, A. Devor, D. Kuzum. 2018. "Deep 2-photon imaging and artifact-free optogenetics through transparent graphene microelectrode arrays." Nat Commun, Volume 9, Issue 1, pp. 2035 - ?. https://doi.org/10.1038/s41467-018-04457-5
Abstract
Recent advances in optical technologies such as multi-photon microscopy and optogenetics have revolutionized our ability to record and manipulate neuronal activity. Combining optical techniques with electrical recordings is of critical importance to connect the large body of neuroscience knowledge obtained from animal models to human studies mainly relying on electrophysiological recordings of brain-scale activity. However, integration of optical modalities with electrical recordings is challenging due to generation of light-induced artifacts. Here we report a transparent graphene microelectrode technology that eliminates light-induced artifacts to enable crosstalk-free integration of 2-photon microscopy, optogenetic stimulation, and cortical recordings in the same in vivo experiment. We achieve fabrication of crack- and residue-free graphene electrode surfaces yielding high optical transmittance for 2-photon imaging down to ~ 1 mm below the cortical surface. Transparent graphene microelectrode technology offers a practical pathway to investigate neuronal activity over multiple spatial scales extending from single neurons to large neuronal populations.
Description
License
© The Author(s) 2018. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/