A platform for brain-wide imaging and reconstruction of individual neurons
Files
Date
2016-01-20
Authors
Economo, Michael N.
Clack, Nathan G.
Lavis, Luke D.
Gerfen, Charles R.
Svoboda, Karel
Myers, Eugene W.
Chandrashekar, Jayaram
Version
Published version
OA Version
Citation
Michael N Economo, Nathan G Clack, Luke D Lavis, Charles R Gerfen, Karel Svoboda, Eugene W Myers, Jayaram Chandrashekar. 2016. "A platform for brain-wide imaging and reconstruction of individual neurons.." Elife, Volume 5:e10566. https://doi.org/10.7554/eLife.10566
Abstract
The structure of axonal arbors controls how signals from individual neurons are routed within the mammalian brain. However, the arbors of very few long-range projection neurons have been reconstructed in their entirety, as axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue. We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data. We demonstrate the power of this approach by reconstructing the axonal arbors of multiple neurons in the motor cortex across a single mouse brain.
Description
License
This is an open-access article,
free of all copyright, and may be
freely reproduced, distributed,
transmitted, modified, built
upon, or otherwise used by
anyone for any lawful purpose.
The work is made available under
the Creative Commons CC0
public domain dedication.