Evaluation of the mouse eye as a model for recurrent herpetic infection
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Abstract
The eyes, the trigeminal ganglia and the superior
cervical ganglia of CD-1 mice were studied as a model for
recurrent herpetic infections. Animals were corneally infected
with Type 1 herpes simplex virus (HSV-1). During the period
of acute infection, both gnaglionic tissues were demonstrated
to have almost 100% infection with herpes virus. Following
recovery from the acute infection, HSV was shown to be latent
in both ganglionic tissues; the HSV could be demonstrated in
over 75% of trigeminal ganglia and approximately 20% of
superior cervical ganglia by explantation of gnaglia. During
t he latent phase, spontaneous reactivation in both ocular
and ganglionic t issues were uncommon in our animals. Different
stimuli, including fever, thermal burn, mechanical trauma,
treatment with cyclophosphamide (CPA), an immunosuppressant,
and physical stress were employed in attempts to induce
recurrent infection. Daily cultures of eye swabbings and
cultures of homogenates of the ganglia were used to sutdy
effects of these stimuli on virus reactivation. A low
incidence of reactivation (approximately 10%) was demonstrated
in eyes and trigeminal gnaglia following CPA trea t men~ of
these mice. The other stimuli apparently p~oduced no reactivation
of infection in our animals. This mouse eye model may
still prove to be useful for studying the mechaniams of viral latency and reactivation in herpetic infection, and thus warrants investigation.
Description
Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1982 (Oral biology)
Bibliography: leaves 102-114.
Bibliography: leaves 102-114.
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