Physiological concentration of human salivary histatins in glandular secretions and whole saliva
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Abstract
Histatins are a group of histidine-rich antimicrobial proteins present
in human salivary secretions. Previous studies have shown that histatins
play an important role in the maintenance of enamel integrity and defense
against oral bacteria and fungal pathogens in the oral cavity. Because
histatins differ in their ability to inhibit blastopore viability and germ tube
formation, it is important to be able to quantitatively determine individual
histatin concentrations. Such determinations would be essential for
understanding and comparing the antibacterial activities of the saliva in
healthy and diseased persons, and in finding a relation between individual
histatin concentration and function.
The present study is focused on determination of the physiological
concentration of the major histatins 1, 3, and 5 in glandular secretions and
whole saliva in order to evaluate the relationship of individual histatin
concentrations to their functional capacity in the oral cavity. Human parotid
and submandibular / sublingual secretions were collected from 19 healthy
donors in the presence and absence of gustatocy stimulation. Whole saliva
was collected from the same group of donors with masticatocy stimulation.
A cationic polyaccylamide gel electrophoresis system (cationic PAGE) was
used in combination with scanning densitometcy to measure the [TRUNCATED].
Description
Includes bibliography: (leaves 114-124).
Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1996 (Oral Biology).
Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1996 (Oral Biology).
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