Prevalence of tetracycline resistance in veillonella; initial studies on developing a genetic system for veilonella
Date
2003
DOI
Authors
Al-Motlaq, Ibrahim M.
Henry M Goldman School of Dental Medicine (Boston University)
Version
OA Version
Citation
Abstract
Veillonella spp. are Gram-negative cocci that have been found to be amongst
the most numerous anaerobes in human saliva. Veillonella spp. have an
unusual physiology and special energy-conservation mechanisms that
facilitate their growth in dental plaque. Unfortunately, the lack of a system
for genetic manipulation of Veillonellae has severely hindered studies of this
interesting group of organisms. In order to begin development of useful
vectors, studies were carried out to identify antibiotic resistance genes from
Veillonella spp. A pBluescript SK (-) phagemid lambda ZAP II library from
Veillonella atypica PK1910 (TetR) was screened by plating on LB agar
containing tetracycline. A phagemid conferring tetracycline resistance was
isolated .. The phagemid contained a 6.6 kb DNA insert.The entire sequence
of the insert was determined and it contained nine ORFs. All of the open
reading frames were homologous to similar ORFs from the conjugative
transposon Tn916 from Enterococcus faecalis including the tetracycline
resistance gene, tetM. [TRUNCATED]
Description
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Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2003 (Pediatric Dentistry).
Includes bibliographical references (leaves 96-113).
Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2003 (Pediatric Dentistry).
Includes bibliographical references (leaves 96-113).
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.