Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
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Accepted manuscript
First author draft
Date
2019-09-19
Authors
Winnubst, Johan
Bas, Erhan
Ferreira, Tiago A.
Wu, Zhuhao
Economo, Michael N.
Edson, Patrick
Arthur, Ben J.
Bruns, Christopher
Rokicki, Konrad
Schauder, David
Version
Accepted manuscript
OA Version
Citation
Johan Winnubst, Erhan Bas, Tiago A Ferreira, Zhuhao Wu, Michael N Economo, Patrick Edson, Ben J Arthur, Christopher Bruns, Konrad Rokicki, David Schauder, Donald J Olbris, Sean D Murphy, David G Ackerman, Cameron Arshadi, Perry Baldwin, Regina Blake, Ahmad Elsayed, Mashtura Hasan, Daniel Ramirez, Bruno Dos Santos, Monet Weldon, Amina Zafar, Joshua T Dudman, Charles R Gerfen, Adam W Hantman, Wyatt Korff, Scott M Sternson, Nelson Spruston, Karel Svoboda, Jayaram Chandrashekar. 2019. "Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain.." Cell, Volume 179, Issue 1, pp. 268 - 281.e13. https://doi.org/10.1016/j.cell.2019.07.042
Abstract
Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons constitute more than 85 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.
Description
Published in final edited form as: Cell. 2019 September 19; 179(1): 268–281.e13. doi:10.1016/j.cell.2019.07.042.