Studies on lipid metabolism and acid mucopolysaccharides in healthy and diseased canine gingivae
Date
1971
DOI
Authors
Lawrence, Felix R.
Version
OA Version
Citation
Abstract
A study was made on healthy and diseased canine gingival tissue
to determine certain aspects of lipid and acid mucopolysaccharide
biochemistry.
Lipids were separated by thin-layer chromatography and the phospholipids
determined by colorimetry, the neutral lipids by gas-liquid
chromatography. The acid mucopolysaccharides {AMPS} were measured by
densitometry after separation by electrophoresis. Tissue samples were
also incubated for 4 hours with [l-1~C]-acetate. The incorporation
into various lipid classes was determined by using liquid scintillation
counting. The study revealed that both total lipids and total AMPS
were decreased during gingival disease. Total AMPS were also distinguished
as hyaluronic acid and sulfated AMPS (chondroitin sulfate A, B
and C and heparitin sulfate}. Hyaluronic acid showed a decrease in
diseased tissue while the sulfated AMPS were increased. The level of
incorporation of [1 ~C] into the lipid classes was decreased in diseased
gingiva.
On the basis of these findings it appears that a decrease in lipids
and AMPS may- be characteristic of gingival disease. The decreased incorporation
of [1 ~C] suggests a decrease in lipid synthesis in the
diseased state. The significance of these findings has been discussed.
Description
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Thesis (M.Sc.D.)--Boston University, School of Graduate Dentistry, 1971 (Oral biology)
Bibliography included.
Thesis (M.Sc.D.)--Boston University, School of Graduate Dentistry, 1971 (Oral biology)
Bibliography included.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.