Effects of phosphorus on proliferation and differentiation of human dental pulp cells
Date
2013
DOI
Authors
Baiz, Adriana
Version
OA Version
Citation
Abstract
Previous studies have demonstrated that the concentration of phosphorus combined with calcium and silicon maximizes proliferation, differentiation, and mineralization in human osteoblasts and dental pulp cells. The main objective of this study was to compare and determine the effects of various specific combinations and concentrations of phosphorus, calcium, and silicon ions against the isolated effects of phosphorus ions on human dental pulp cell proliferation and mineralization. Dental pulp tissue for this study was obtained from freshly extracted third molars of patients in the 15-20 year age range. Tissue samples were collected from wisdom teeth indicated for extraction, without any carious lesions, restorations, or any evidence of pathology. Undifferentiated mesenchymal cells were isolated and cultured from these tissue samples. They were then exposed to: (A) 100 ppm silicon, 33 ppm calcium, 16 ppm phosphorus; (B) 50 ppm silicon, 16 ppm calcium, 8 ppm phosphorus; (C) 16 ppm phosphorus; and (D) 8 ppm phosphorus. Cell attachment, proliferation, and mineralization rates were measured at 16 hours, 7 days, and 14 days. After analyzing the preliminary results using one-way ANOVA and Tukey HSD Test at a 95% confidence interval, no statistica11y significant difference was found in the attachment or proliferation rates between the different groups at various time points; however, it demonstrated to significantly increase ALP expression. Within the limitations of this study, it can be concluded that at its highest concentration, ion phosphorus significantly increased ALP activity on human dental pulp cells in vitro.
Description
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Thesis (MSD) --Boston University, Goldman School of Dental Medicine, 2013 (Department of Endodontics).
Includes bibliography: leaves 70-78.
Thesis (MSD) --Boston University, Goldman School of Dental Medicine, 2013 (Department of Endodontics).
Includes bibliography: leaves 70-78.
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.