Calculating DNA yield from different direct lysis procedures on blood deposited onto various fabrics
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Citation
Abstract
The goal of forensic DNA analysis is to develop a profile from an item of evidence to create linkages in a crime. To generate an interpretable short tandem repeat (STR) profile, purified Deoxyribonucleic Acid (DNA) must be extracted from the cell.Silica-binding extractions consist of several purification steps that yield Polymerase Chain Reaction (PCR)-ready DNA in the solution. However, these purification steps introduce the potential for loss of DNA. Direct lysis methods eliminate the potential for loss of DNA since extraction can be done in one tube. Conversely, since direct lysis methods do not contain a purification step, the resulting extracted DNA lysate may contain inhibitors that prevent successful PCR analysis.
Samples retrieved from crime scenes are typically poor or degraded. Blood is a body fluid commonly encountered at crime scenes or on items of evidence, however; it contains many PCR inhibitors that affect downstream DNA analysis.
The purpose of this study is to evaluate DNA recovery from whole blood deposited on cotton, nylon, wool, rayon, or cotton swabs using several available direct lysis reagents.
Evaluation and comparison of four direct lysis reagents are reported.
Description
2024