The effects of silicon, calcium and magnesium on survivin expression of normal human osteoblasts
Date
2011
DOI
Authors
Ortiz, Jan Nomar
Version
OA Version
Citation
Abstract
Survivin is a recently discovered anti-apoptotic molecule from the IAP family. It is involved in cell mitosis process and has been related to cancerous tumors. Although negative connotations of survivin are usually emphasized in most studies, this project directs the attention to its positive characteristic: its assistance during proliferation to human osteoblasts. Objectives: This study intends to verify if survivin exopression can be manipulated using external factors such as silicon, calcium and magnesium.
Methods: Normal human osteoblast cell cultures derived from human donors were cultured and placed in well plates at a concentration of 1.5 x 10[5] cells. Cells were cultured with essential medium as a control and with medium containing supplemental silicon, calcium and magnesium ions at a concentration of 50 ppm, 30 ppm and 100 ppm respectively. Cell attachment, cell proliferation, mineralization, alkaline phosphatase (ALP) activity, osteocalcin and survivin expression were measured at different timeframes.
Results: Compared with the control group, the Ca 30ppm and Mg 100ppm groups presented an increase of survivin expression at all three time intervals while the Si 50ppm group presented a decrease of survivin expression at all timeframes. Interestingly, survivin expression in the Ca 30ppm group was significantly greater than the Si 50ppm and the control groups at all timeframes (P[less than] 0.05). A greater cell proliferation rate was observed in all study groups at both timeframes, but it was significantly higher only in the Ca 30ppm group (P[less than] 0.05]. Mineralization was greater in the Ca 30ppm and Si 50 ppm groups compared to the control group only at 21 days (P[less than]0.05). Alkaline Phosphatase activity was significantly greater in all study groups at 21 days but not at 7 days (P[less than]0.05). Osteocalcin expression was greater in all study groups at both time intervals, but only significantly greater in the Ca 30 ppm group at 21 days (P[less than]0.05).
Conclusion: This study demonstrated that survivin expression could be significantly up regulated by enhanced normal human osteoblast cultures, resulting in up regulated cell proliferation. Mineralization was greater in the Ca 30ppm and Si 50ppm groups compared to the control group only at 21 days (P[less than]0.05).Alkaline Phosphatase activity was significantly greater in all study groups at 21 days but not at 7 days (P[less than]0.05). Osteocalcin expression was greater in all study groups at both time intervals, but only significantly greater in the Ca 30 ppm group at 21 days (P[less than]0.05).
Conclusion: This study demonstrated that survivin expression could be significantly up regulated by enhanced normal human osteoblast cultures, resulting in up regulated cell proliferation.
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Thesis (DScD) --Boston University, Goldman School of Dental Medicine, 2011 (Department of Orthodontics and Dentofacial Orthopedics).
Includes bibliographic references: leaves 135-155.
Thesis (DScD) --Boston University, Goldman School of Dental Medicine, 2011 (Department of Orthodontics and Dentofacial Orthopedics).
Includes bibliographic references: leaves 135-155.
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.