Ueda, Hiroki R.Dodt, Hans-UlrichOsten, PavelEconomo, Michael N.Chandrashekar, JayaramKeller, Philipp J.2023-10-122023-10-122020-05-06H.R. Ueda, H.-U. Dodt, P. Osten, M.N. Economo, J. Chandrashekar, P.J. Keller. 2020. "Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy." Neuron, Volume 106, Issue 3, pp.369-387. https://doi.org/10.1016/j.neuron.2020.03.0040896-62731097-4199https://hdl.handle.net/2144/46930Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.369-387PrintenNeurosciencesPsychologyCognitive sciencesNeurology & neurosurgeryBiological psychologyAnimalsBrainHumansImaging, three-dimensionalMicroscopyOptical imagingStaining and labelingArticle2023-03-0610.1016/j.neuron.2020.03.0040000-0001-9893-1505 (Economo, Michael N)561868