Economo, Michael N.Winnubst, JohanBas, ErhanFerreira, Tiago A.Chandrashekar, Jayaram2019-09-032019-09-032019-09-01Michael N Economo, Johan Winnubst, Erhan Bas, Tiago A Ferreira, Jayaram Chandrashekar. 2019. "Single-neuron axonal reconstruction: The search for a wiring diagram of the brain.." J Comp Neurol, Volume 527, Issue 13, pp. 2190 - 2199. https://doi.org/10.1002/cne.246741096-9861https://hdl.handle.net/2144/37643Reconstruction of the axonal projection patterns of single neurons has been an important tool for understanding both the diversity of cell types in the brain and the logic of information flow between brain regions. Innovative approaches now enable the complete reconstruction of axonal projection patterns of individual neurons with vastly increased throughput. Here, we review how advances in genetic, imaging, and computational techniques have been exploited for axonal reconstruction. We also discuss how new innovations could enable the integration of genetic and physiological information with axonal morphology for producing a census of cell types in the mammalian brain at scale.Reconstruction of the axonal projection patterns of single neurons has been an important tool for understanding both the diversity of cell types in the brain and the logic of information flow between brain regions. Innovative approaches now enable the complete reconstruction of axonal projection patterns of individual neurons with vastly increased throughput. Here, we review how advances in genetic, imaging, and computational techniques have been exploited for axonal reconstruction. We also discuss how new innovations could enable the integration of genetic and physiological information with axonal morphology for producing a census of cell types in the mammalian brain at scale.2190 - 2199Axonal reconstructionLong-range circuitsWhole-brain imagingNeurosciencesZoologyMedical physiologyNeurology & neurosurgeryArticle10.1002/cne.24674452792