Yaseen, Mohammad A.Sakadzic, SavaWu, WeichengBecker, WolfgangKasischke, Karl A.Boas, David A.2019-09-102019-09-102013-02-01Mohammad A Yaseen, Sava Sakadzic, Weicheng Wu, Wolfgang Becker, Karl A Kasischke, David A Boas. 2013. "In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH." BIOMEDICAL OPTICS EXPRESS, Volume 4, Issue 2, pp. 307 - 321 (15). https://doi.org/10.1364/BOE.4.0003072156-7085https://hdl.handle.net/2144/37768Minimally invasive, specific measurement of cellular energy metabolism is crucial for understanding cerebral pathophysiology. Here, we present high-resolution, in vivo observations of autofluorescence lifetime as a biomarker of cerebral energy metabolism in exposed rat cortices. We describe a customized two-photon imaging system with time correlated single photon counting detection and specialized software for modeling multiple-component fits of fluorescence decay and monitoring their transient behaviors. In vivo cerebral NADH fluorescence suggests the presence of four distinct components, which respond differently to brief periods of anoxia and likely indicate different enzymatic formulations. Individual components show potential as indicators of specific molecular pathways involved in oxidative metabolism.307 - 321 (15)© 2013 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.Life sciences & biomedicineBiochemical research methodsOpticsRadiology, nuclear medicine & medical imagingBiochemistry & molecular biologyMedical optics and biotechnologyLifetime-based sensingNonlinear microscopyArticle10.1364/BOE.4.0003070000-0002-6709-7711 (Boas, David A)241769