Scott, Benjamin B.Thiberge, Stephan Y.Guo, CaiyingTervo, Dougal G.R.Brody, Carlos D.Karpova, Alla Y.Tank, David W.2022-04-192022-04-192018B.B. Scott, S.Y. Thiberge, C. Guo, D.G.R. Tervo, C.D. Brody, A.Y. Karpova, D.W. Tank. 2018. "Imaging Cortical Dynamics in GCaMP Transgenic Rats with a Head-Mounted Widefield Macroscope." Neuron, Volume 100, Issue 5, pp. 1045 - 1058. https://doi.org/10.1016/j.neuron.2018.09.050.0896-6273https://hdl.handle.net/2144/44248Widefield imaging of calcium dynamics is an emerging method for mapping regional neural activity but is currently limited to restrained animals. Here we describe cScope, a head- mounted widefield macroscope developed to image large-scale cortical dynamics in rats during natural behavior. cScope provides a 7.8 by 4 mm field of view, dual illumination paths for both fluorescence and hemodynamic correction, and can be fabricated at low cost using readily attainable components. We also report the development of Thy-1 transgenic rat strains with widespread neuronal expression of the calcium indicator GCaMP6f. We combined these two technologies to image large-scale calcium dynamics in the dorsal neocortex during a visual evidence accumulation task. Quantitative analysis of task-related dynamics revealed multiple regions having neural signals that encode behavioral choice and sensory evidence. Our results provide a new transgenic resource for calcium imaging in rats and extend the domain of headmounted microscopes to larger-scale cortical dynamics.p. 1045 - 1058en-USAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Articlehttps://doi.org/10.1016/j.neuron.2018.09.050404315