Barboza, Eliane dos Santos PortoHenry M Goldman School of Graduate Dentistry (Boston University)2018-10-312018-10-3119901990(OCoLC)41341182b22385915https://hdl.handle.net/2144/31740PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please log in with a valid BU account to access and click Download. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1990 (Periodontology).Includes bibliographical references: (leaves 52-55).Protein glycosylation at the asparagine residues (Nglycosylation) is obligatory for eukaryotic cell function. The early genes in the N-glycosylation pathway encode glycosyltransferases that catalyze the synthesis of the precursor oligosaccharide. To elucidate how this synthesis is regulated three early genes, ALG7, ALGl and ALG2, of the N-linked glycosylation pathway were analyzed in the yeast§..:.. cerevisiae. Transcripts of these genes were examined in yeast cells grown under different nutritional conditions to exponential and stationary phases. The results indicate that, in the exponential phase, cells grown in minimal media under glucose deprivation have the highest steady-state levels for all the three gene transcripts, while in stationary phase, the highest steady-state levels were observed when cells were grown under optimal conditions. Also, the steady-state transcript levels of ALG7, ALGl and ALG2 are, at least, an order of magnitude higher in the exponentially growing cells as compared to the stationary. The differences are most obvious for the ALG7 gene. We have also investigated the involvement of the ALG7 gene in the physiology of the hamster parotid gland. [TRUNCATED]en-USThis work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.GlycoproteinsEukaryotic cellsRegulation of the early genes in the N-linked glycosylation pathwayThesis/Dissertation99195984700001161