Pearre, BenMichas, ChristosTsang, Jean-MarcGardner, TimOtchy, Timothy2019-09-202019-09-202018-02-26Ben Pearre, Christis Michas, Jean-Marc Tsang, Tim Gardner, Timothy Otchy. "Fast Micron-Scale 3D Printing with a Resonant-Scanning Two-Photon Microscope." arxiv, Issue arXiv:1803.07135v1,https://hdl.handle.net/2144/379733D printing allows rapid fabrication of complex objects from digital designs. One 3D-printing process, direct laser writing, polymerises a light-sensitive material by steering a focused laser beam through the shape of the object to be created. The highest-resolution direct laser writing systems use a femtosecond laser to effect two-photon polymerisation. The focal (polymerisation) point is steered over the shape of the desired object with mechanised stages or galvanometer-controlled mirrors. Here we report a new high-resolution direct laser writing system that employs a resonant mirror scanner to achieve a significant increase in printing speed over galvanometer- or piezo-based methods while maintaining resolution on the order of a micron. This printer is based on a software modification to a commerically available resonant-scanning two-photon microscope. We demonstrate the complete process chain from hardware configuration and control software to the printing of objects of approximately 400×400×350μm, and validate performance with objective benchmarks. Released under an open-source license, this work makes micro-scale 3D printing available the large community of two-photon microscope users, and paves the way toward widespread availability of precision-printed devices.en-USApplied physicsInstrumentation and detectorsArticle443736