Christadore, Lisa M.Grinstaff, Mark W.Schaus, Scott E.2020-05-012020-05-012018-04-01Lisa Christadore, Mark W Grinstaff, Scott E Schaus. 2018. "Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection." MOLECULES, Volume 23, Issue 4,10 pp. (10). https://doi.org/10.3390/molecules230409361420-3049https://hdl.handle.net/2144/40515Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available.10 pagesen-USThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedhttp://creativecommons.org/licenses/by/4.0/Science & technologyLife sciences & biomedicinePhysical sciencesBiochemistry & molecular biologyChemistry, multidisciplinaryDendrimerHydrogelScreeningCrosslinkingFluorescenceBiomedical applicationsCatalyzed oxidationsPamam dendrimerDrug-deliveryNucleic acidsWound closureIn-vitroNitronesAminesAdhesivesMicroscopy, confocalPolyethylene glycolsSerum albumin, bovineSingle-cell analysisSpectrometry, fluorescenceOrganic chemistryTheoretical and computational chemistryMedicinal and biomolecular chemistryArticle10.3390/molecules230409360000-0002-5453-3668 (Grinstaff, Mark W)364246