Wang, TianyuOuzounov, Dimitre G.Wu, ChunyanHorton, Nicholas G.Zhang, BinWu, Cheng-HsunZhang, YanpingSchnitzer, Mark J.Xu, Chris2024-05-312024-05-312018-10T. Wang, D.G. Ouzounov, C. Wu, N.G. Horton, B. Zhang, C.-H. Wu, Y. Zhang, M.J. Schnitzer, C. Xu. 2018. "Three-photon imaging of mouse brain structure and function through the intact skull." Nature Methods, Volume 15, Issue 10, pp.789-792. https://doi.org/10.1038/s41592-018-0115-y1548-70911548-7105https://hdl.handle.net/2144/48942Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and imaging system. Here we demonstrate three-photon imaging of vasculature through the adult mouse skull at >500-μm depth, as well as GCaMP6s calcium imaging over weeks in cortical layers 2/3 and 4 in awake mice, with 8.5 frames per second and a field of view spanning hundreds of micrometers.p. 789-792Print-ElectronicenBiological sciencesTechnologyMedical and health sciencesDevelopmental biologyAnimalsBrainFemaleImage processing, computer-assistedMaleMiceMice, inbred C57BLMicroscopy, fluorescence, multiphotonNeuroimagingSkullArticle2024-03-2010.1038/s41592-018-0115-y0000-0002-6087-6376 (Wang, Tianyu)0000-0002-3493-6427 (Xu, Chris)914107