Avci, OguzhanUnlu, Nese LortlarOzkumur, Ayca YalcinUnlu, M. Selim2020-04-132020-04-132015-07-01Oguzhan Avci, Nese Lortlar Unlu, Ayca Yalcin Ozkumur, M Selim Unlu. 2015. "Interferometric Reflectance Imaging Sensor (IRIS)-A Platform Technology for Multiplexed Diagnostics and Digital Detection." SENSORS, Volume 15, Issue 7, pp. 17649 - 17665 (17). https://doi.org/10.3390/s1507176491424-8220https://hdl.handle.net/2144/40125Over the last decade, the growing need in disease diagnostics has stimulated rapid development of new technologies with unprecedented capabilities. Recent emerging infectious diseases and epidemics have revealed the shortcomings of existing diagnostics tools, and the necessity for further improvements. Optical biosensors can lay the foundations for future generation diagnostics by providing means to detect biomarkers in a highly sensitive, specific, quantitative and multiplexed fashion. Here, we review an optical sensing technology, Interferometric Reflectance Imaging Sensor (IRIS), and the relevant features of this multifunctional platform for quantitative, label-free and dynamic detection. We discuss two distinct modalities for IRIS: (i) low-magnification (ensemble biomolecular mass measurements) and (ii) high-magnification (digital detection of individual nanoparticles) along with their applications, including label-free detection of multiplexed protein chips, measurement of single nucleotide polymorphism, quantification of transcription factor DNA binding, and high sensitivity digital sensing and characterization of nanoparticles and viruses.p. 17649 - 17665en-USThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Science & technologyPhysical sciencesChemistry, analyticalElectrochemistryInstruments & instrumentationChemistryOptical biosensorInterferometryLabel-freeDigital detectionNanoparticleVirusSingle nucleotide polymorphismSingle nanoparticle detectionWhispering-gallery-modeBiomolecular interactionsVirus detectionDNABiosensorsHybridizationMicroarraysAdsorptionBiosensing techniquesDiagnostic imagingHumansSignal processing, computer-assistedElectrical and electronic engineeringEnvironmental science and managementEcologyArticle10.3390/s1507176490000-0002-8594-892X (Unlu, M Selim)41080