Boassa, DanielaLemieux, Sakina P.Lev-Ram, VardaHu, JunruXiong, QingPhan, SebastianMackey, MasonRamachandra, RanjanPeace, Ryan E.Adams, Stephen R.Ellisman, Mark H.Ngo, John T.2020-01-092020-01-092019-10-17Daniela Boassa, Sakina Lemieux, Varda Lev-Ram, Junru Hu, Qing Xiong, Sebastian Phan, Mason Mackey, Ranjan Ramachandra, Ryan Peace, Stephen Adams, Mark Ellisman, and John T. Ngo. 2019. "Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopy." Cell Chemical Biology, Volume 26, Issue 10, Pages 1407-1416.e5. https://doi.org/10.1016/j.chembiol.2019.07.0072451-9456https://hdl.handle.net/2144/39061Please note: this work is indefinitely embargoed in OpenBU. No public access is forecasted. To request access, please click on the lock icon and fill out the appropriate web form.A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as “multicolor” electron microscopy (EM) via the photooxidation of 3-3’-diaminobenzidine (DAB) and its derivatives.Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopyArticle10.1016/j.chembiol.2019.07.0070000-0003-3508-1915 (Ngo, John)475208