Xie, HongyuChung, David Y.Kura, SreekanthSugimoto, KazutakaAykan, Sanem A.Wu, YiSakadžić, SavaYaseen, Mohammad A.Boas, David A.Ayata, Cenk2021-05-062021-05-062020-04Hongyu Xie, David Y Chung, Sreekanth Kura, Kazutaka Sugimoto, Sanem A Aykan, Yi Wu, Sava Sakadžić, Mohammad A Yaseen, David A Boas, Cenk Ayata. 2020. "Differential effects of anesthetics on resting state functional connectivity in the mouse.." J Cereb Blood Flow Metab, Volume 40, Issue 4, pp. 875 - 884. https://doi.org/10.1177/0271678X198471231559-7016https://hdl.handle.net/2144/42499Blood oxygen level-dependent (BOLD) functional MRI (fMRI) is a standard approach to examine resting state functional connectivity (RSFC), but fMRI in animal models is challenging. Recently, functional optical intrinsic signal imaging-which relies on the same hemodynamic signal underlying BOLD fMRI-has been developed as a complementary approach to assess RSFC in mice. Since it is difficult to ensure that an animal is in a truly resting state while awake, RSFC measurements under anesthesia remain an important approach. Therefore, we systematically examined measures of RSFC using non-invasive, widefield optical intrinsic signal imaging under five different anesthetics in male C57BL/6J mice. We find excellent seed-based, global, and interhemispheric connectivity using tribromoethanol (Avertin) and ketamine-xylazine, comparable to results in the literature including awake animals. Urethane anesthesia yielded intermediate results, while chloral hydrate and isoflurane were both associated with poor RSFC. Furthermore, we found a correspondence between the strength of RSFC and the power of low-frequency hemodynamic fluctuations. In conclusion, Avertin and ketamine-xylazine provide robust and reproducible measures of RSFC in mice, whereas chloral hydrate and isoflurane do not.p. 875 - 884en-USCopyright Author(s) 2019. Article reuse guidelines: https://sagepub.com/journals-permissionsAnesthesiaAnimal modelsBasic scienceBrain imagingCerebral hemodynamicsAnestheticsAnimalsBlood pressureBrainBrain mappingMagnetic Resonance ImagingMaleMiceMice, Inbred C57BLOxygenWakefulnessCardiorespiratory medicine and haematologyClinical sciencesNeurosciencesNeurology & neurosurgeryArticle10.1177/0271678X198471230000-0002-6709-7711 (Boas, David A)475390