Zhu, LijiaQi, JiChiao, Christine Ya-ChiZhang, QiangPorco, John A.Faller, Douglas V.Dai, Yan2018-01-252018-01-252014-11Zhu, l., Qi, J., Chiao, C.Y., Zhang, Q., Porco Jr, J.A., Faller, d.V., & Dai, Y. (2014). Identification of a novel polyprenylated acylphloroglucinol‑derived SIRT1 inhibitor with cancer‑specific anti-proliferative and invasion-suppressing activities. International Journal of Oncology, 45, 2128-2136. https://doi.org/10.3892/ijo.2014.26391791-2423https://hdl.handle.net/2144/26414SIRT1, a class III histone deacetylase, plays a critical role in regulating cancer cell growth, migration and invasion, which makes it a potential target for cancer therapeutics. In this study, we screened derivatives of several groups of natural products and identified a novel SIRT1 inhibitor JQ-101, a synthetic derivative of the polyprenylated acylphloroglucinol (PPAP) natural products, with an IC(50) for SIRT1 of 30 µM in vitro, with 5-fold higher activity for SIRT1 vs. SIRT2. Exposure of tumor cells to JQ-101 significantly enhanced acetylation of p53 and histone H4K16 at known sites of SIRT1 deacetylation, validating SIRT1 as its cellular target. JQ-101 suppressed cancer cell growth and survival by targeting SIRT1, and also exhibited selective cytotoxicity towards a panel of human tumor cell lines, while producing no toxicity in two normal human cell types at comparable concentrations. JQ-101 induced both apoptosis and cell senescence, and suppressed cancer cell invasion in vitro. In summary, we have identified JQ-101 as a new SIRT1 inhibitor which may have potential application in cancer treatment through its ability to induce tumor cell apoptosis and senescence and suppress cancer cell invasion.p. 2128 - 2136Life sciences & biomedicineOncologySIRT1 inhibitorPolyprenylated acylphloroglucinolCancer cell growthCancer cell invasionSmall-molecule inhibitorProstate cancerCell migrationBreast cancerLung metastasisDown-regulationPoor prognosisDU145 cellsApoptosisCell movementCell proliferationCellular senescenceGene expression regulation, neoplasticHumansNeoplasm invasivenessNeoplasmsPhloroglucinolSirtuin 1Sirtuin 2Cell agingPolyprenylated acylphloroglucinolCancer cell growthCancer cell invasionOncology and carcinogenesisArticle10.3892/ijo.2014.263925189993