Uhlirova, HanaKılıç, KıvılcımTian, PeifangThunemann, MartinDesjardins, MichèleSaisan, Payam A.Sakadžić, SavaNess, Torbjørn V.Mateo, CelineCheng, QunWeldy, Kimberly L.Razoux, FlorenceVandenberghe, MatthieuCremonesi, Jonathan A.Ferri, Christopher G.L.Nizar, KrystalSridhar, Vishnu B.Steed, Tyler C.Abashin, MaximFainman, YeshaiahuMasliah, EliezerDjurovic, SrdjanAndreassen, Ole A.Silva, Gabriel A.Boas, David A.Kleinfeld, DavidBuxton, Richard B.Einevoll, Gaute T.Dale, Anders M.Devor, Anna2019-09-102019-09-102016-05-31Hana Uhlirova, Kıvılcım Kılıç, Peifang Tian, Martin Thunemann, Michèle Desjardins, Payam A Saisan, Sava Sakadžić, Torbjørn V Ness, Celine Mateo, Qun Cheng, Kimberly L Weldy, Florence Razoux, Matthieu Vandenberghe, Jonathan A Cremonesi, Christopher Gl Ferri, Krystal Nizar, Vishnu B Sridhar, Tyler C Steed, Maxim Abashin, Yeshaiahu Fainman, Eliezer Masliah, Srdjan Djurovic, Ole A Andreassen, Gabriel A Silva, David A Boas, David Kleinfeld, Richard B Buxton, Gaute T Einevoll, Anders M Dale, Anna Devor. 2016. "Cell type specificity of neurovascular coupling in cerebral cortex.." Elife, Volume 5, https://doi.org/10.7554/eLife.143152050-084Xhttps://hdl.handle.net/2144/37776Identification of the cellular players and molecular messengers that communicate neuronal activity to the vasculature driving cerebral hemodynamics is important for (1) the basic understanding of cerebrovascular regulation and (2) interpretation of functional Magnetic Resonance Imaging (fMRI) signals. Using a combination of optogenetic stimulation and 2-photon imaging in mice, we demonstrate that selective activation of cortical excitation and inhibition elicits distinct vascular responses and identify the vasoconstrictive mechanism as Neuropeptide Y (NPY) acting on Y1 receptors. The latter implies that task-related negative Blood Oxygenation Level Dependent (BOLD) fMRI signals in the cerebral cortex under normal physiological conditions may be mainly driven by the NPY-positive inhibitory neurons. Further, the NPY-Y1 pathway may offer a potential therapeutic target in cerebrovascular disease.Copyright Uhlirova et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.http://creativecommons.org/licenses/by/4.0/2-photon microscopyNPYConstrictionDilationMouseNeuroscienceOptogeneticAnimalsCerebral cortexCerebrovascular disordersDiagnostic imagingGene expressionMagnetic resonance imagingMaleMiceMice, transgenicNeuronsNeuropeptide YNeurovascular couplingOptogeneticsOrgan specificityOxygenPhotic stimulationProtein bindingReceptors, neuropeptide YVasoconstrictionVasoconstrictor agentsArticle10.7554/eLife.143150000-0002-6709-7711 (Boas, David A)241762