Kwong, GeorgeMarquez, Hector A.Yang, ChianWong, Joyce Y.Kotton, Darrell N.2022-08-042022-08-042019-09-10G. Kwong, H.A. Marquez, C. Yang, J.Y. Wong, D.N. Kotton. 2019. "Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering.." Stem Cell Reports, Volume 13, Issue 3, pp. 499 - 514. https://doi.org/10.1016/j.stemcr.2019.07.0142213-6711https://hdl.handle.net/2144/44982Induced pluripotent stem cells (iPSCs) provide a potential source for the derivation of smooth muscle cells (SMCs); however, current approaches are limited by the production of heterogeneous cell types and a paucity of tools or markers for tracking and purifying candidate SMCs. Here, we develop murine and human iPSC lines carrying fluorochrome reporters (Acta2hrGFP and ACTA2eGFP, respectively) that identify Acta2+/ACTA2+ cells as they emerge in vitro in real time during iPSC-directed differentiation. We find that Acta2hrGFP+ and ACTA2eGFP+ cells can be sorted to purity and are enriched in markers characteristic of an immature or synthetic SMC. We characterize the resulting GFP+ populations through global transcriptomic profiling and functional studies, including the capacity to form engineered cell sheets. We conclude that these reporter lines allow for generation of sortable, live iPSC-derived Acta2+/ACTA2+ cells highly enriched in smooth muscle lineages for basic developmental studies, tissue engineering, or future clinical regenerative applications.p. 499 - 514en-US© 2019 The Author(s). This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.http://creativecommons.org/licenses/by/4.0/Article10.1016/j.stemcr.2019.07.014487129