Rodríguez, CristinaChen, AndersonRivera, José A.Mohr, Manuel A.Liang, YajieNatan, Ryan G.Sun, WenzhiMilkie, Daniel E.Bifano, Thomas G.Chen, XiaokeJi, Na2025-02-062025-02-062021-10C. Rodríguez, A. Chen, J.A. Rivera, M.A. Mohr, Y. Liang, R.G. Natan, W. Sun, D.E. Milkie, T.G. Bifano, X. Chen, N. Ji. 2021. "An adaptive optics module for deep tissue multiphoton imaging in vivo." Nature Methods, Volume 18, Issue 10, pp.1259-1264. https://doi.org/10.1038/s41592-021-01279-01548-70911548-7105https://hdl.handle.net/2144/49717Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.pp. 1259-1264Print-Electronicen-USTechnologyMedical and health sciencesDevelopmental biologyBiological sciencesAnimalsBacterial proteinsEmbryo, nonmammalianFemaleGreen fluorescent proteinsLuminescent proteinsMaleMiceNeuroimagingOptics and photonicsZebrafishArticle10.1038/s41592-021-01279-00000-0002-5525-3174 (Rodríguez, Cristina)0000-0001-6110-5285 (Chen, Anderson)0000-0002-5189-541X (Mohr, Manuel A)0000-0002-3123-4833 (Natan, Ryan G)0000-0002-3917-6965 (Milkie, Daniel E)0000-0003-4952-2515 (Bifano, Thomas G)0000-0002-5527-1663 (Ji, Na)644071