Kılıç, KıvılcımDesjardins, MichèleTang, JianboThunemann, MartinSunil, SmrithiErdener, Sefik EvrenPostnov, Dmitry D.Boas, David A.Devor, Anna2021-12-202021-12-202020K. Kılıç, M. Desjardins, J. Tang, M. Thunemann, S. Sunil, Ş.E. Erdener, D.D. Postnov, D.A. Boas, A. Devor. 2020. "Chronic Cranial Windows for Long Term Multimodal Neurovascular Imaging in Mice.." Front Physiol, Volume 11, 612678. https://doi.org/10.3389/fphys.2020.6126781664-042Xhttps://hdl.handle.net/2144/43491Chronic cranial windows allow for longitudinal brain imaging experiments in awake, behaving mice. Different imaging technologies have their unique advantages and combining multiple imaging modalities offers measurements of a wide spectrum of neuronal, glial, vascular, and metabolic parameters needed for comprehensive investigation of physiological and pathophysiological mechanisms. Here, we detail a suite of surgical techniques for installation of different cranial windows targeted for specific imaging technologies and their combination. Following these techniques and practices will yield higher experimental success and reproducibility of results.en-USCopyright © 2021 Kılıç, Desjardins, Tang, Thunemann, Sunil, Erdener, Postnov, Boas and Devor. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.http://creativecommons.org/licenses/by/4.0/Article10.3389/fphys.2020.612678602767