Xiao, ShengTseng, Hua-anGritton, HowardHan, XueMertz, Jerome2020-01-162020-01-162018-05-21Sheng Xiao, Hua-an Tseng, Howard Gritton, Xue Han, Jerome Mertz. 2018. "Video-rate volumetric neuronal imaging using 3D targeted illumination." SCIENTIFIC REPORTS, Volume 8, pp. ? - ? (10). https://doi.org/10.1038/s41598-018-26240-82045-2322https://hdl.handle.net/2144/39106Fast volumetric microscopy is required to monitor large-scale neural ensembles with high spatio-temporal resolution. Widefield fluorescence microscopy can image large 2D fields of view at high resolution and speed while remaining simple and costeffective. A focal sweep add-on can further extend the capacity of widefield microscopy by enabling extended-depth-of-field (EDOF) imaging, but suffers from an inability to reject out-of-focus fluorescence background. Here, by using a digital micromirror device to target only in-focus sample features, we perform EDOF imaging with greatly enhanced contrast and signal-to-noise ratio, while reducing the light dosage delivered to the sample. Image quality is further improved by the application of a robust deconvolution algorithm. We demonstrate the advantages of our technique for in vivo calcium imaging in the mouse brain.10 p.en-USAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Science & technologyMultidisciplinary sciencesExtended depthField microscopyIn-vivoFocusLiveAlgorithmsAnimalsBrainFemaleFluorescenceImage processing, computer-assistedLightingMice, inbred C57BLMicroscopy, fluorescenceNeuroimagingVideo recordingBiochemistry and cell biologyArticle10.1038/s41598-018-26240-8389251