De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite
Hoffman, Joseph Ivan
Sorenson, Michael D.
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Citation (published version)Martina Pauli, Nayden Chakarov, Oliver Rupp, Jörn Kalinowski, Alexander Goesmann, Michael D Sorenson, Oliver Krüger, Joseph Ivan Hoffman. 2015. "De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite.." BMC Genomics, Volume 16, pp. 1038 - ?. doi:10.1186/s12864-015-2254-1
BACKGROUND: Studies of non-model species are important for understanding the molecular processes underpinning phenotypic variation under natural ecological conditions. The common buzzard (Buteo buteo; Aves: Accipitriformes) is a widespread and common Eurasian raptor with three distinct plumage morphs that differ in several fitness-related traits, including parasite infestation. To provide a genomic resource for plumage polymorphic birds in general and to search for candidate genes relating to fitness, we generated a transcriptome from a single dead buzzard specimen plus easily accessible, minimally invasive samples from live chicks. RESULTS: We not only de novo assembled a near-complete buzzard transcriptome, but also obtained a significant fraction of the transcriptome of its malaria-like parasite, Leucocytozoon buteonis. By identifying melanogenesis-related transcripts that are differentially expressed in light ventral and dark dorsal feathers, but which are also expressed in other regions of the body, we also identified a suite of candidate genes that could be associated with fitness differences among the morphs. These include several immune-related genes, providing a plausible link between melanisation and parasite load. qPCR analysis of a subset of these genes revealed significant differences between ventral and dorsal feathers and an additional effect of morph. CONCLUSION: This new resource provides preliminary insights into genes that could be involved in fitness differences between the buzzard colour morphs, and should facilitate future studies of raptors and their malaria-like parasites.
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