A preliminary investigation into the chemical nature of the Rh antigens
Everhart, Donald Lee
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The inhibition technique was used in an attempt to determine the chemical structure of Rh antigens. A saline agglutinating Rh antibody was mixed with a chemical compound of known structure, containing carbohydrate, and believed to be similar in configuration to the Rh antigen in question. This mixture was first incubated, then the appropriate erythrocytes were added. Following a second incubation, the degree of agglutination was read in the usual manner for Rh testing. A saline control was carried out for each experiment. If the unknown showed less agglutination than the control, then the chemical compound was said to have an inhibitory effect. In all these experiments the inhibitor was diluted and not the antibody. The three Rh antibodies, anti-C, anti-D, and anti-E, were tested with the same carbohydrates, D- and L-glucose, D- and L-mannose, D-ribose, and L-lyxose. The antibodies were also tested with antibiotics which included streptomycin, stylomycin, carbomycin, and two degradation products of the first two, streptobiosamine and 6-dimethyl-amino-9-(3'amino-31deoxy-beta-D-ribofuranosyl)-purine. Some of the above carbohydrates and streptobiosamine were coupled with aniline and p-aminophenol, and then tested as inhibitors. 5'adenylic acid and 2', 3'adenylic acid were also tested as inhibitors. Their reactions suggested the use of indol acetic acid and indol butyric acid. Two amino acids, tyrosine and phenylalanine, were tested. In addition, paminophenyl acetic acid and salicin were also used as inhibitors. [TRUNCATED]
Thesis (Ph.D.)--Boston University.