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    Multi-contrast imaging and digital refocusing on a mobile microscope with a domed LED array

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    License
    Attribution 4.0 International
    Date Issued
    2015
    Publisher Version
    10.1371/journal.pone.0124938
    Author(s)
    Tian, Lei
    Sadras, Nitin
    Waller, Laura
    Phillips, Zachary F.
    D'Ambrosio, Michael V.
    Rulison, Jared J.
    Patel, Hurshal S.
    Gande, Aditya V.
    Switz, Neil A.
    Fletcher, Daniel A.
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    Permanent Link
    https://hdl.handle.net/2144/25611
    Citation (published version)
    Zachary F. Phillips, Michael V. D'Ambrosio, Lei Tian, Jared J. Rulison, Hurshal S. Patel, Nitin Sadras, Aditya V. Gande, Neil A. Switz, Daniel A. Fletcher, Laura Waller. 2015. "Multi-contrast imaging and digital refocusing on a mobile microscope with a domed LED array.." PLoS One, v. 10, Issue 5, pp. e0124938 1-13.
    Abstract
    We demonstrate the design and application of an add-on device for improving the diagnostic and research capabilities of CellScope--a low-cost, smartphone-based point-of-care microscope. We replace the single LED illumination of the original CellScope with a programmable domed LED array. By leveraging recent advances in computational illumination, this new device enables simultaneous multi-contrast imaging with brightfield, darkfield, and phase imaging modes. Further, we scan through illumination angles to capture lightfield datasets, which can be used to recover 3D intensity and phase images without any hardware changes. This digital refocusing procedure can be used for either 3D imaging or software-only focus correction, reducing the need for precise mechanical focusing during field experiments. All acquisition and processing is performed on the mobile phone and controlled through a smartphone application, making the computational microscope compact and portable. Using multiple samples and different objective magnifications, we demonstrate that the performance of our device is comparable to that of a commercial microscope. This unique device platform extends the field imaging capabilities of CellScope, opening up new clinical and research possibilities.
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    Attribution 4.0 International
    Collections
    • BU Open Access Articles [3866]
    • ENG: Electrical and Computer Engineering: Scholarly Papers [265]


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