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dc.contributor.authorHuang, George T.-J.en_US
dc.date.accessioned2018-09-18T15:34:09Z
dc.date.available2018-09-18T15:34:09Z
dc.date.issued1992
dc.date.submitted1992
dc.identifier.other(OCoLC)38558465
dc.identifier.otherb21870196
dc.identifier.urihttps://hdl.handle.net/2144/31297
dc.descriptionThesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1992 (Oral Biology).en_US
dc.descriptionIncludes bibliographical references (leaves 70-84).en_US
dc.description.abstractThe ALG7 gene encodes the tunicamycin-sensitive, dolichol-P-dependent Nacetylglucosamine- 1-phosphate transferase, GPT, that catalyzes the synthesis of the first dolichollinked sugar, Dol-PP-GlcNAc, in the N-glycosylation pathway. ALG7 has been evQlutionarily conserved and is essential for growth in all eukaryotes. The ALG7 gene expression in yeast is known to be regulated in part by the 3' untranslated regions (UTR) of the ALG7 multiple transcripts at the posttranscriptional level. To examine the regulatory features of the mammalian ALG7 gene, cloning and characterization of the hamster ALG7 mRNAs were undertaken. Polymerase chain reaction (PCR) using a single ALG7 gene-specific primer was performed to clone the cDNAs corresponding to the 3' and 5' ends of the ALG7 mRNAs from the Chinese hamster ovary (CHO) cells. The initial Northern blot analysis using a hamster ALG7 genomic DNA as a probe has shown that in the CHO cells the ALG7 gene is transcribed into three major messages, approximately 1.5, 1.9, and 2.2 kb in size. The 1.9 kb transcripts were cloned and sequenced. There is one consensus polyadenylation signal AAUAAA located 12 nucleotides (nt) upstream to the major poly(A) site. Three additional minor poly(A) sites are located at 18, 21 and 29 nt downstream from the AAUAAA sequence in this 1.9 kb class of mRNAs. [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.en_US
dc.subjectEukaryotic cellsen_US
dc.titleMolecular cloning and characterization of multiple transcripts of the hamster ALG7 geneen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameDoctor of Science in Dentistryen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineOral Biologyen_US
etd.degree.grantorBoston Universityen_US


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