The ALG-7 gene expression in developing hamster submandibular gland
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Asaparagine-linked glycosyaltion is essential for eukaryotic l'ell growth and changes in this function have profound effects on cell physiology. Previous study have shown that in the yeast S. cerevisiae, which shares the early steps of N-glyc-osylation with higher eukaryotes, the activity of N-glycosylation function is regulated at the level of transcription at the very first step in the pathway catalyzed by UDPGlcNAC: Dol-P GlcNAc-Ptransferase encoded by the ALG-7 gene. To examine the molecular controls of N-glycosyaltion and assess their involvement in development we have studied transcriptional expression of the ALG- 7 gene in the postnatally developing hamster salivary submandibular gland. The hamster submandibular gland was surgically removed at 4, 12, 24, and 72 days after birth and poly-(A) + RNAs isolated. The ALG-7 specific transcripts were quantified with a Slot blot procedure using a fragment of hamster ALG- 7 cDNA labeled with 32P-dCTP by nick translation. Our result indicate that at the early stages of postnatal development , 4 and 12 days, the steady-state level of ALG-7 mRNA me ~-fold higher than in the adult. At 24 days the level of the ALG-7 mRNA decre ases to 4-fold over the adult level. therefore, changes in the ALG-7 expression at the level of transcription accompany postnatal development of the submandibular gland.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1991.Includes bibliography (leaves 33-36)
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