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dc.contributor.authorYaseen, Mohammad A.en_US
dc.contributor.authorSakadzic, Savaen_US
dc.contributor.authorWu, Weichengen_US
dc.contributor.authorBecker, Wolfgangen_US
dc.contributor.authorKasischke, Karl A.en_US
dc.contributor.authorBoas, David A.en_US
dc.date.accessioned2019-09-10T17:13:52Z
dc.date.available2019-09-10T17:13:52Z
dc.date.issued2013-02-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000314806600013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e74115fe3da270499c3d65c9b17d654
dc.identifier.citationMohammad A Yaseen, Sava Sakadzic, Weicheng Wu, Wolfgang Becker, Karl A Kasischke, David A Boas. 2013. "In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH." BIOMEDICAL OPTICS EXPRESS, Volume 4, Issue 2, pp. 307 - 321 (15). https://doi.org/10.1364/BOE.4.000307
dc.identifier.issn2156-7085
dc.identifier.urihttps://hdl.handle.net/2144/37768
dc.description.abstractMinimally invasive, specific measurement of cellular energy metabolism is crucial for understanding cerebral pathophysiology. Here, we present high-resolution, in vivo observations of autofluorescence lifetime as a biomarker of cerebral energy metabolism in exposed rat cortices. We describe a customized two-photon imaging system with time correlated single photon counting detection and specialized software for modeling multiple-component fits of fluorescence decay and monitoring their transient behaviors. In vivo cerebral NADH fluorescence suggests the presence of four distinct components, which respond differently to brief periods of anoxia and likely indicate different enzymatic formulations. Individual components show potential as indicators of specific molecular pathways involved in oxidative metabolism.en_US
dc.description.sponsorshipThis work was supported by NIH R01-NS057476, P50-NS010828, and P01-NS055104, R01-EB000790 and AHA 11SDG7600037. We gratefully acknowledge Boston Electronics for their generous support with the TCSPC equipment and software. Dr. Becker has a financial interest in Becker & Hickl GmbH, which did not support this work. (R01-NS057476 - NIH; P50-NS010828 - NIH; P01-NS055104 - NIH; R01-EB000790 - NIH; 11SDG7600037 - AHA)en_US
dc.format.extent307 - 321 (15)en_US
dc.languageEnglish
dc.publisherOPTICAL SOC AMERen_US
dc.relation.ispartofBIOMEDICAL OPTICS EXPRESS
dc.rights© 2013 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.en_US
dc.subjectLife sciences & biomedicineen_US
dc.subjectBiochemical research methodsen_US
dc.subjectOpticsen_US
dc.subjectRadiology, nuclear medicine & medical imagingen_US
dc.subjectBiochemistry & molecular biologyen_US
dc.subjectMedical optics and biotechnologyen_US
dc.subjectLifetime-based sensingen_US
dc.subjectNonlinear microscopyen_US
dc.titleIn vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADHen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1364/BOE.4.000307
pubs.elements-sourceweb-of-scienceen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0002-6709-7711 (Boas, David A)
dc.identifier.mycv241769


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