Kinetics of the human salivary proteins onto synthetic hydroxyapatite
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Dental pellicle, the acellular organic film which is deposited on the tooth surface after eruption or cleaning, is of interest with regard to its chemical composition and mechanism of formation. One interaction of particular interest is the selective adsorption of certain salivary proteins to dental enamel. The mineral in enamel, primarily calcium and phosphate, consist predominantly of hydroxyapatite crystals. Since hydroxyapatite is the major component of tooth enamel, synthetic preparations of hydroxyapatite were used as a substitute for enamel on pellicle formation. Parotid and submandibular/sublingual secretions were adsorbed onto hydroxyapatite at a ratio of 1 ml saliva per 5 mg adsorbent for various times (0, 1(1), 2(1) 5(1),(10(1),30(1), and 6(1). The adsorbed and unadsorbed fractions were analyzed by both anionic and cationic discontinuous polyacrylamide gel electrophoresis and amino acid analysis. Acidic proline-rich proteins, statherin, and histatin 1, histatin 3 and histatin 5 were identified from the parotid-derived pellicle. The submandibular/sublingual- derived pellicle consists of the same proteins, but in addition, it contained cystatins. Also, the adsorption of acidic proline-rich proteins, statherin and to a lesser extent salivary cystatins were found to be time-dependent. These proteins are mainly adsorbed during the first 15 minutes of exposure to hydroxyapatite. A significant difference was found for salivary histatins, which over the time studied, demonstrated a slower rate of adsorption. This study indicated that the adsorption of salivary proteins is highly selective and that acidic phosphoproteins, as well as basic proteins show selective adsorption for hydroxyapatite and, therefore, may be involved in the initial stages of pellicle formation.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1990 (Periodontology and Oral Biology)Includes bibliography (leaves 72-82)
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