Long-term hiPSC-derived motor neuron culture conditions
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Amyotrophic Lateral Sclerosis (ALS), which is also referred to as Charcot disease or Lou Gehrig’s disease, is a fatal and incurable neuromuscular disorder that affects motor neurons. Developing a long-term hiPSC-derived motor neuron culture system helps analyze and visualize age-related phenomenon that would not be apparent in typical shorter-term culture systems. Ren-VM Cells were used prior to hiPSCs to demonstrate that protein localization and mislocalization associated with ALS can be analyzed. A novel hiPSC-derived motor neuron maintenance protocol was developed to generate ideal conditions for imaging and analyzing protein localization and mislocalization. Ultimately, a novel replating protocol was developed, which generates hiPSC-derived motor neuronal cultures that are mature and can be analyzed effectively.
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