Osteogenic material for bone tissue engineering
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It has been a challenge to identify the materials with desirable bio-solubility and osteogenic activity for bone tissue engineering scaffolds. This study was designed to test the bioactivity of a novel composite of polymer and bioactive glass in vitro for bone regeneration. In this study, bioactive glass was included to stimulate osteogenesis, while the synthetic polymer was used to provide mechanical strength. Two groups of scaffold wafers (100:0 and 20:80 vol. PLGA:bioglass) 2 mm thick and 10 mm in diameter, were fabricated by melt molding. Normal human osteoblasts were extracted from the alveolar bone of young healthy adults, and expanded to the second passage for experiments. Cells were screened for osteoblast phenotype prior to all experiments, seeded onto scaffold wafers in triplicate at a concentration of 1.5 × 10 [superscipt 4] cells/cm [superscript 2], and cultured for 16 hours, 7 days, and 14 days. Cell attachment efficiency at 16 hours post seeding was examined. At 7 days post seeding, cell proliferation rate was evaluated. Alkaline phosphatase (ALP) activity was determined by the measurement of pNP mixture in a fixed cellular layer at day 7. The expression of osteocalcin by the cultures were measured at three different time intervals. Von Kossa staining was used to evaluate calcium deposit by the cultures at 14 days. SEM was performed at 3 different time intervals. All data were calibrated on a per cell basis and tested by ANOVA and Scheffe statistical analysis. The results showed that composite wafers of bioglass and PLGA yielded no significant difference in cell attachment efficiency, but significantly higher levels of osteoblast cell proliferation, osteocalcin expression, alkaline phosphatase activity, and mineralization. This in vitro study indicated that a composite of bioglass and PLGA may have the potential to stimulate the osteophenotype of cultured human osteoblasts. Further study is needed to establish the in vitro and in vivo profile of this osteogenic composite material for bone tissue engineering.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (M.Sc.D)--Boston University, Henry M. Goldman School of Dental Medicine, 2001 (Orthodontics).Includes bibliographical references (leaves 112-126).
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