Boston University Libraries OpenBU
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    •   OpenBU
    • Theses & Dissertations
    • Boston University Theses & Dissertations
    • View Item
    •   OpenBU
    • Theses & Dissertations
    • Boston University Theses & Dissertations
    • View Item

    Reverberation multiphoton microscopy for volumetric imaging in scattering media

    Thumbnail
    Date Issued
    2020
    Author(s)
    Beaulieu, Devin R.
    Share to FacebookShare to TwitterShare by Email
    Export Citation
    Download to BibTex
    Download to EndNote/RefMan (RIS)
    Metadata
    Show full item record
    Permanent Link
    https://hdl.handle.net/2144/41019
    Abstract
    Multiphoton microscopy has become an extremely valuable tool for peering deeply into thick, scattering media such as biological tissue. However, the traditional multiphoton beam-scanning approach is held back because only one thin plane is observed at a time. The reverberation loop elegantly overcomes this limitation by generating an infinite series of foci at depths spanning the sample, all sampled individually but near-simultaneously. With the inclusion of some additional interleave steps, it is possible to quickly scan a sample at video rates – allowing volumetric imaging at or near the rate one would traditionally image planes. In neural imaging, this enables a reverberation multiphoton microscope to simultaneously monitor relationships in neuronal activity not only horizontally across samples, but vertically across many layers of the brain. In imaging of engineered cardiac tissues, this enables high resolution observation of three-dimensional structures in a live sample, even as it actively beats and moves.
    Collections
    • Boston University Theses & Dissertations [6914]


    Boston University
    Contact Us | Send Feedback | Help
     

     

    Browse

    All of OpenBUCommunities & CollectionsIssue DateAuthorsTitlesSubjectsThis CollectionIssue DateAuthorsTitlesSubjects

    Deposit Materials

    LoginNon-BU Registration

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Boston University
    Contact Us | Send Feedback | Help