Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy
Files
Accepted manuscript
Date
2020-05-06
Authors
Ueda, Hiroki R.
Dodt, Hans-Ulrich
Osten, Pavel
Economo, Michael N.
Chandrashekar, Jayaram
Keller, Philipp J.
Version
Accepted manuscript
OA Version
Citation
H.R. Ueda, H.-U. Dodt, P. Osten, M.N. Economo, J. Chandrashekar, P.J. Keller. 2020. "Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy." Neuron, Volume 106, Issue 3, pp.369-387. https://doi.org/10.1016/j.neuron.2020.03.004
Abstract
Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.