Bacteriology of lesions of endodontic origin
Date
[1984]
DOI
Authors
Chemel, David
Version
OA Version
Citation
Abstract
The purpose of this investigation was to determine if there is bacterial invasion of periapical tissues of symptomatic and asymptomatic endodontically involved teeth.
Seven patients were selected with lesions of endodontic origin or an acute alveolar abcess. A surgical approach was used to remove tissue from the lesions of endodontic origin.
As contamination from the oral mucosa was a major concern, two methods of surface decontamination were tested:
a) Swabbing the mucosa with alcohol followed by metaphen.
b) A 2% chlorhexidine rinse.
The tissue sample was handled using strict anaerobic techniques. The tissue was ground in a tissue grinder under a constant flow of oxygen free gas, sonicated and then plated in different dilutions on Trypticase Soy agar. The plates were incubated anaerobically and the predominant organisms characterized using an extensive series of biochemical tests including gram staining, oxygen tolerance, fermentations and hydrolysis reactions.
Results were recorded on a Tektronix computer with the aid of a prompted data entry program. Isolates were identified using a probablistic identification program. Organisms were cultured from six of the seven samples. No organisms were cultured from the AAA. 51.7% of the isolates were anaerobes. The samples fell into two groups depending upon the disinfectant used. The alcohol and metaphen group were mostly gram positive streptococci and facultative rods. The chlorhexidine group consisted only of gram negative rods of which 86.2% were anaerobic. The dominant species isolated were B. oris and 2 groups of gram negative rods which did not fit existing species descriptions. This is the first description of isolating B. gracilis and “fusiform” Bacteroides from endodontic lesions.
Description
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Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1984, (Endodontics)
Bibliography: leaves 84-90.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1984, (Endodontics)
Bibliography: leaves 84-90.
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.